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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2008 Oct 7;46(1):33–41. doi: 10.1016/j.freeradbiomed.2008.09.022

Figure 3. Cytoplasmic p21 prevents the loss Mcl-1 and Bcl-XL during hyperoxia.

Figure 3

H1299 cells with inducible expression of EGFp21 or EGFp21ΔNLS were cultured in the absence (−) or presence (+) of doxycyline (DOX) for 24 hrs then immediately harvested (day 0) or exposed to hyperoxia for 2 and 4 days. (A) Protein was isolated from cells and immunoblotted for expression of p21, Mcl-1, Bcl-XL, and actin was used as a loading control and densitometry was performed for (B) Mcl-1 and (C) Bcl-XL. Immunoblots shown are representative of at least three separate experiments with similar results.