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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2008 Oct 7;46(1):33–41. doi: 10.1016/j.freeradbiomed.2008.09.022

Figure 5. Expression of p21 rescues hyperoxic down-regulation of BiP.

Figure 5

H1299 cells with inducible expression of EGFp21 were cultured in the absence (−) or presence (+) of doxycyline (DOX) for 24 hrs and exposed room air (day 0) or hyperoxia for 2 and 4 days. (A) Protein lysates were isolated and immunoblotted for EGFp21, BiP, CHOP and actin was used as a loading control. EGFp21 inducible cells were cultured in room air (day 0) or hyperoxia for 2 and 4 days. Doxycycline was introduced at different times during exposure to induce EGFp21 expression with −1 corresponding to 1 day prior to hyperoxia exposure, 0 corresponding to the time of exposure and +1 and +2 corresponding to 1 day and 2 days into hyperoxia respectively. (B) Protein lysates were harvested and immunoblotted for EGFp21, BiP, CHOP and actin was used as a loading control. Immunoblots are representative of three separate experiments with similar results.