Table 2.
ssDNA sequences of the oligonucleotides used in padlocks, target molecules and primers (5'-3' orientation).
| Name | 5' target | cZIP sequence | 3' target | Target gene (GenBank:) | Size (nt) |
| Cotton sp. | CTGGGCTGAGAACAACATTCTGACTCACCTCAAACCA | AAGTGTGCCAGACGCTCGAA | CTTTAAATCTTTGGAGGG | [AJ132636] (sad1) | 122 |
| Maize sp. | CTGTGGCATCATCACTGGCATCGT | GTACTACATTCGTGCGATGG | TTAGGCGTCATCAT | [AF371266] (zein) | 124 |
| Canola sp. | GTGACGCATACGTTCTATAACATCAGCCTGTCC | CGTCGCGTTAGACAGCTCAT | CCGATCTTTCTTGTATTC | [DQ173668] (ACCase) | 118 |
| Soya sp. | GCATCATAGGTAATGAGAACCTTGGCTACTTTATTGTTGGCC | ACTCCAGTGCCAAGTACGAT | AGAGGCTGGTGGAG | [K00821] (lectin1) | 142 |
| RRS event | GATCCCAAATAGTTTTGTTTTTCTAACAACGAGAAGCTATATGTAGATGCTATT | AACAACGATGAGACCGGGCT | TCAAACAGTTCTTCTCC | [AJ308515] | 138 |
| MON810 | GGCAATGGCAAAGGATGTTAAACGTTAGAGTCCTTCGT | TGCCCTATTGTTGCGTCGGA | AAAGTGACAGATAGCTG | [AF434709] | 141 |
| Bt176 event | GAACATCAGATCTCGGTGACGGGCAGGACC | TAATCTAATTCTGGTCGCGG | GCATCAATGGAGGAGA | [AJ878607] | 113 |
| CaMV P35S | CGAAGGATAGTGGGATTGTGCGTCATCCCTTACG | CCACGAGCTGTAATCCGGTA | ATAGAGGAAGGGTCTTG | [V00141] | 118 |
| FMV P35S | TCTTCGGTGGATGTCTTTTTCTGAAACTTACTGACCATGATG | GTGATTAAGTCTGCTTCGGC | GCCCACTAACTTTAAG | [X06166] | 125 |
| bar | TCGATGTAGTGGTTGACGATGGTGCAGACCG | CGAGTGCTCCGTGCGAAATA | TGACCGTGCTTGTC | [AY346130] | 112 |
| SpikeLock | CGTCGGACAGGTTACTTTCGAAGAGCCGGAATACTC | GCTGAGGTCGATGCTGAGGTCGCA (cZIP-P for Positioning) | CGAAGGTCATATCTCG | 123 | |
| ZB3 | CGTGCAAGTTACCGAGCTGA | 20 | |||
| primer sequence | |||||
| Fwd primer | GCAAGAGATGGGCTACAGAGGAT | 23 | |||
| Fwd25 primer | CCGCAAGAGATGGGCTACAGAGGAT | 25 | |||
| Rev primer | GGACAGACACGCTAAGACAGAACT | 24 |