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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Mol Cell Biochem. 2008 Nov 11;322(1-2):63–71. doi: 10.1007/s11010-008-9940-0

Fig. 2.

Fig. 2

The Δ315–316 mutant is not detected on immunoblot with the BXP-21 antibody. Membrane proteins extracted from HEK 293 cells stably transfected with the wild type (482R) and the mutants (Q141K, Δ315–316, and Q141K/D315–316) were separated on SDS/PAGE (18 μg/lane), transferred onto a PVDF membrane, and incubated overnight with the mouse monoclonal anti-ABCG2 antibody BXP-21 (upper panel) or the 405 rabbit polyclonal anti-ABCG2 antibody (lower panel). Two clones are shown for the Q141K mutant and three clones for the Δ315–316 and Q141K/Δ315–316 mutants, with clone numbers indicated. Membranes were stained with 0.1% Ponceau S and checked for comparable loading (not shown)