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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Stroke. 2008 Dec 18;40(2):618–625. doi: 10.1161/STROKEAHA.108.524447

Figure 2.

Figure 2

Mitochondrial upregulation of PUMA preceded cytochrome c release and caspase-9 activation. A, Western blot analysis showed that immunoreactivity of PUMA in the cytosolic fraction was only slightly detectable at any time point. In contrast, PUMA immunoreactivity significantly increased in the mitochondrial fraction 4 and 24 hours after tGCI (n=4, *P<0.05). β-Actin and COX IV analyses are shown as internal controls. c, control; OD, optical density. B, Western blot analysis showed that nuclear p53 was significantly increased 4 hours after tGCI (n=4, *P<0.05). TFIID analysis is shown as an internal control. C, Western blot analysis revealed that cytosolic cytochrome c and cleaved caspase-9 were significantly increased in the hippocampal CA1 subregion 24 hours after tGCI (n=4, *P<0.05). β-Actin analysis is shown as an internal control.