. Author manuscript; available in PMC: 2009 Nov 15.

Published in final edited form as: Bioorg Med Chem. 2008 Sep 30;16(22):9764–9771. doi: 10.1016/j.bmc.2008.09.063

Table 1.

Decomposition of JS-K and its structural analogues in the presence of GSH and GSH/GST.

			GSTP1-1		GSTA1-1		GSTM1-1
Compd	moles of NO released^a	half-life (min)^b	specific activity^c (µmol·mg⁻¹min⁻¹)	relative rate	specific activity^c (µmol·mg⁻¹min⁻¹)	relative rate	specific activity^c (µmol·mg⁻¹min⁻¹)	relative rate
JS-K	1.7	30	1.36	1	66.3	1	126	1
3a	1.0	44	1.15	0.85	55.5	0.84	112	0.89
3b	1.3	29	0.88	0.65	47.0	0.71	110	0.87
3c	1.75	26	1.17	0.86	70.3	1.06	172	1.36
3d	1.7	26	0.14	0.10	17.6	0.27	49	0.39
4a	2.0	52	0.16	0.12	16.8	0.25	38	0.30
4b	1.35	30	0.98	0.72	45.1	0.68	102	0.81
4c	2.0	27	1.2	0.88	59.0	0.89	126.3	1.0

Determined by measuring NO release from the compound (100–150 nM) in the presence of glutathione (1 mM) in 0.1 M pH 7.4 phosphate buffer at 37 °C by chemiluminescence analysis.

Determined by spectrophotometric analysis of the compound (33 µM) in the presence of glutathione (1 mM) in pH 7.4 phosphate buffered saline at 37 °C. Half-life is calculated by fitting the rate of decomposition to a first order process.

Determined by spectrophotometric analysis of the compound (50 µM) in the presence of GSH/GST at 37 °C