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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Future Lipidol. 2008 Dec;3(6):637–649. doi: 10.2217/17460875.3.6.637

Figure 3. Configuration of the 4E6 sandwich-type and competition ELISA.

Figure 3

The sandwich-type ELISA uses ox-LDL-specific monoclonal antibody 4E6 as the capturing antibody and the anti-ApoB100-specific antibody 8A2 as the tagging antibody. The latter is conjugated with HRP, which reacts with a specific substrate to yield a yellow-colored reaction product; this is quantified in the spectrophotometer. The competition ELISA requires preincubation of the plasma sample with 4E6. The sample is then applied to a microtiter plate, on which in vitro ox-LDL is immobilized. There, the ox-LDL in the plasma and the in vitro ox-LDL compete for 4E6. After washing, 4E6 bound to the immobilized ox-LDL is detected with HRP conjugated rabbit-anti-mouse antibodies. The reaction is completed as in the sandwich-type ELISA. HRP: Horseradish peroxidase; ox-LDL: Oxidized LDL.