FIGURE 10.
Effects of selective caspase inhibition on LCA- and ent-LCA-induced caspase activation in HT-29 cells. Selective inhibitors of caspase-2 and caspase-8, a pan-caspase inhibitor, or vehicle control (CTRL, Me2SO) were used as pretreatment for HT-29 cells treated with 500 μm LCA for 1 h (A) and HT-29 cells treated with 500 μm ent-LCA for 1 h (B). In all of the experiments caspase inhibitor treated cells were pretreated with 50 μm Z-VDVAD-FMK (2 indicates caspase-2 inhibitor), Z-IETD-FMK (8 indicates caspase-8 inhibitor), or Z-VAD-FMK (Pan indicates pan-caspase inhibitor) for 4 h prior to bile acid exposure.