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Reactive oxygen species generation in response to natural and enantiomeric LCA. HT-29 (A) and HCT-116 (B) cells pretreated with CM-H2DCFDA were exposed to 500 μm LCA or ent-LCA or control medium for either 5, 10, or 30 min. After the indicated time the amount of ROS was determined, and the values are reported as fold increases over untreated control cells. For all of the experiments the values are reported as the means ± S.D. (n = 3). *, p < 0.05; #, p < 0.07.
