Caspase-3 and caspase-9 activation in HT-29 and HCT-116 cells following
treatment with natural and enantiomeric bile acids. HT-29 and HCT-116
cells were treated for 1 h with vehicle control (CTRL, EtOH) and 500
μm LCA and ent-LCA (A), 500 μm
CDCA and ent-CDCA (B), or 500 μm DCA and
ent-DCA (C). In all of the experiments, the attached and detached
cells were collected, and active and inactive caspase-3 and caspase-9 were
probed.