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. 2009 Jan 30;284(5):3354–3364. doi: 10.1074/jbc.M805804200

FIGURE 4.

FIGURE 4.

Caspase-3 and caspase-9 activation in HT-29 and HCT-116 cells following treatment with natural and enantiomeric bile acids. HT-29 and HCT-116 cells were treated for 1 h with vehicle control (CTRL, EtOH) and 500 μm LCA and ent-LCA (A), 500 μm CDCA and ent-CDCA (B), or 500 μm DCA and ent-DCA (C). In all of the experiments, the attached and detached cells were collected, and active and inactive caspase-3 and caspase-9 were probed.