FIGURE 5.
Proliferation of HT-29 and HCT-116 cells in response to natural and enantiomeric LCA. A, effects of varying concentrations (10, 50, 100, 250, and 500 μm) of LCA and ent-LCA on proliferation. All of the concentrations were applied for 48 h. B, time course analysis of HT-29 and HCT-116 proliferation in response to 250 μm LCA or ent-LCA at 24, 48, and 72 h. In all of the experiments, proliferation is determined by the hexosaminidase assay and is relative to vehicle-treated control (EtOH) (n ≥ 3). The values are expressed as the means ± S.E. *, p < 0.05.