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Determination of Ki and kinact based on the method of Kitz and Wilson (50). Shown is a double-reciprocal plot of the apparent rate constants of inhibition (kapp; slopes of Fig. 4) versus the reciprocal of inhibitor concentration ([I]). The Ki and kinact values were determined to be 56 μm and 0.1 min–1, respectively, based on the following formula: 1/Kapp = (1/kinact + Ki/kinact) × 1/[I].
