Skip to main content
. 2009 Jan 30;284(5):2598–2602. doi: 10.1074/jbc.C800206200

FIGURE 1.

FIGURE 1.

Phosphorylation of α-synuclein Ser-129 by PLK2. A, phosphorylation of a biotinylated α-synuclein substrate by recombinant PLK1 (▪), PLK2 (•), PLK3 (⋄), PLK4 (□), and CK2 (○). Phosphorylation of the substrate at Ser-129 was measured using a phosphorylation-specific antibody with the kinase assay described under “Experimental Procedures.” Acceptor/donor ratio (A/D ratio) is a ratio of acceptor and donor fluorescence intensities. Inset, acceptor/donor ratios of the p-Ser-129 (pS129) synuclein standard. B and C, reduction of PLK2 mRNA (B) and p-Ser-129 α-synuclein (C) by lentiviral constructs either containing a scrambled sequence (Control) or expressing PLK2 shRNA, in human cortical cultures overexpressing α-synuclein (E46K mutant). The relative multiplicity of infection (MOI) is shown. **, p < 0.01; ***, p < 0.001. Relative copy numbers (copy#) of PLK2 mRNA were determined by qPCR (“Experimental Procedures”) and normalized to glyceraldehyde-3-phosphate dehydrogenase mRNA. Ratios of p-Ser-129 to total α-synuclein were determined by ELISA.