FIGURE 2.

Reduction of p-Ser-129 α-synuclein by inhibition of PLK2. A–D, inhibition of α-synuclein phosphorylation by BI2536 (▪), AMPU (▵), or DMAT (•) by recombinant PLK2 in the in vitro kinase assay (A), in mouse cortical cultures (B), and in HEK293 cells by endogenous kinase (transfected with empty vector) (C) or by transfected PLK2 (D). Levels of p-Ser-129 α-synuclein were measured by in vitro kinase assay (A) or by ELISA (B–D); the percentage of inhibition relative to controls with DMSO vehicle alone is shown.