FIGURE 3.

Reduction of p-Ser-129 α-synuclein by PLK inhibition in vivo and by PLK2 knock-out. A, mice were treated with either vehicle or 30 mg/kg of BI2536, eight mice per group. Levels of total and p-Ser-129 α-synuclein were measured as under “Experimental Procedures.” B, cortical cultures were prepared from a PLK2 knock-out mouse (–/–) or a wild-type littermate control (+/+). Values shown are the means of triplicate cultures from each donor; error bars show standard deviations. C, fraction of α-synuclein phosphorylated in cerebral cortices from a PLK2 knock-out (–/–) and two wild-type control (+/+) 2–3-month-old mice. Each bar represents results from a single brain; error bars represent standard deviation of assay replicates. Total α-synuclein levels were not altered in either cortical cultures or intact brains (data not shown).