TABLE 2.

Oligonucleotides for electrophoretic mobility shift assays Wild type and mutant oligonucleotides were used in gel shift assays. The YR oligonucleotide containing one Y-repeat region (underlined) from the Runx2 P1 promoter was used as the probe for the electrophoretic mobility shift assay. Other oligonucleotides were used as competitors. Sp1-con, Sp1 consensus binding sites; Ets1-con, Ets1 consensus binding sites; Sp1-Mut, Sp1 consensus with mutations (lowercase) in the Sp1 element; Ets1-Mut, Ets1 consensus with mutations (lowercase) in the Ets1 element.

Oligonucleotide	Sequence (5′–3′)
YR	CAGAAGAGAAAGAGGGAGGGGAGGGGAG
YR-M1	CAGAAacaAAAGAGGGAGGGGAGGGGAG
YR-M2	CAGAAGAGAAAGAGtfAGGGGAGGGGAG
YR-M3	CAGAAGAGAAAGAGGGAGGttAGGGGAG
Sp1-con	ATTCGATCGGGGCGGGGCGAGC
Sp1-Mut	ATTCGATCGGttCGGGGCGAGC
Ets1-con	GATCTCGAGCAGGAAGTTCGA
Ets1-Mut	GATCTCGAGCAaGAAGTTCGA