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. Author manuscript; available in PMC: 2009 Sep 11.
Published in final edited form as: J Med Chem. 2008 Aug 15;51(17):5264–5270. doi: 10.1021/jm800045t

Figure 2.

Figure 2

Inhibitory effect of 1 and 2 on m-calpain (A) and μI–II (B). Duplicate assays were performed by adding 1.3 μM (EDANS)-EPLFAERK-(DABCYL) (1), 125 nM calpain (2) and 4 mM CaCl2 (3), then averaged to yield the plots shown. Autolytic inactivation of m-calpain, but not μI–II, was observed when no inhibitor was added (X). Immediately following addition of each inhibitor (4), the increase in fluorescence intensity was attenuated. 1(Y) caused a more distinct attenuation of fluorescence in both the m-calpain and μI–II assays, indicating that it is a more potent inhibitor when compared to 2 (Z).