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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Cell Commun Adhes. 2008 Nov;15(4):333–349. doi: 10.1080/15419060802440377

Figure 2. VE-cadherin levels in HUVEC are maintained in the absence of N-cadherin.

Figure 2

(A) SiRNA sequences targeting either luciferase (lucif. siRNA) or N-cadherin (Nsmrt, N6D, or N25mod) were delivered into HUVEC via electroporation, and cells were plated at confluence. Immunoblot analysis for N-cadherin (left), VE-cadherin (right), and β-actin was carried out 48 hours after plating. Densitometric analysis of immunoblots is shown below. Values for N-cadherin and VE-cadherin are normalized to β-actin and are expressed relative to luciferase control (mean ± SEM; minimum n=3, for N6D P<0.0001, N25mod P=0.0024, Nsmrt P<0.0001). N6D: individual chemically modified sequence purchased from Dharmacon. Nsmrt: N-cadherin siGENOME Smartpools (unmodified) purchased from Dharmacon. N25mod: individual sequence purchased from Ambion, subsequently chemically modified by Dharmacon.