FIG. 3.

Role of IscR in regulating biofilm formation. Strains indicated were grown at 25°C for 24 h in LB. Final planktonic growth was recorded at 600 nm (light gray bars). After washing, staining with crystal violet, and solubilization, final biofilm formation was recorded at 570 nm (dark gray bars). The average of triplicate experiments is shown. For panel C, 0.05% l-arabinose was also added to all strains at the time of inoculation. piscR expresses wild-type IscR and piscR-CTM expresses the C92A-C98A-C104A triple cysteine mutant of IscR. pFWO2 is the empty parent control plasmid.