TABLE 1.

Oligonucleotides and plasmids for tarIJ inactivation experiments

Oligonucleotide or plasmid	Sequence, description, or reference
Oligonucleotides
48(forward)	5′-CCGCGCGGATCCATTTATCAACTAACTAAGCC-3′
48(reverse)	5′-CTCACGCTGCAGATGAGCAAGAGTCAATAGACG-3′
48c(forward)	5′-CGGCCGGGATCCGTAACAGATTTGAAGATTGC-3′
48c(reverse)	5′-ATGCCCCTGCAGACAAACTCTGTAATGGCTGC-3′
49(forward)	5′-TTAATTGGATCCGGTGGA ACTGGCACACGC-3′
49(reverse)	5′-CAGTACCTGCAGAAGGTGAGCACGATTTGG-3′
49c(forward)	5′-CCGCGCGGATCCCAGCCATTTATTAGTAATTGC-3′
49c(reverse)	5′-TCTGGACTGCAGGGACGATAAGCATCAATGGC-3′
spr1148(forward)	5′-GCGCCGCATATGAGAAAGACTAGTAAAATG-3′
spr1148(reverse)	5′-GCCCAAGAGCTCCTCCAGTACTTATACTTCC-3′
spr1149(forward)	5′-GGAGCTCATATGATTTATGCAGGAATTCTTGC-3′
spr1149(reverse)	5′-GCCCGAGGATCCCTAGTCTTTCTCAATCATAC-3′
Plasmids
pJDC9	Reference 9
p48	pJDC9 with an internal fragment of 474 bp of tarJ
p48c	pJDC9 with 33 bp upstream and 459 bp in tarJ
p49	pJDC9 with an internal fragment of 470 bp of tarI
p49c	pJDC9 with 187 bp upstream and 302 bp of tarI