FIG. 10.

Leucine effects on Lrp-DNA interactions. (A) P_lrp, Up-P_hilA, and P_invF were subjected to EMSA in three different reaction conditions (−/−, without leucine/without Lrp; −/+, without leucine/with Lrp (150 nM); and +/+, with leucine [15 mM]/with Lrp [150 nM]). (B) P_invF-Lrp interaction was analyzed in four different leucine concentrations: 0, 1.5, 7.5, and 15 mM. The binding reaction mixtures were resolved in a 5% polyacrylamide gel, stained with SYBR Gold (Invitrogen), and visualized on a UV transilluminator. The images were inverted to intensify the DNA bands. Data are one of similar results from at least two independent experiments.