FIG. 5.
RT-PCR analyses of virulence genes in the wild-type (WT, χ3761), Δlrp (χ9411), and lrp(Con) (χ9448) strains. (A and C) RNA was isolated from each strain and used for RT-PCR. The RT-PCR products were separated in a 1.5% agarose gel, stained with ethidium bromide, and visualized on a UV transilluminator. The images were inverted to intensify the DNA bands. Data are one of two similar RT-PCR tests with two independent RNA isolations. (B) Expression of Lrp in strain χ9449 (Ptrc-lrp and araC PBAD lacI) in the presence (+Ara) or absence (−Ara) of arabinose. Lane M, dual-color prestained protein standards (Bio-Rad).