FIG. 7.

Analysis of proteins in culture supernatants of the wild-type (WT, χ3761), Δlrp (χ9411), and lrp(Con) (χ9448) strains. (A) Bacterial cells were grown in LB broth to mid-exponential phase. Cells were removed by centrifugation and proteins in the culture supernatant were precipitated with trichloroacetic acid (10%), resolved in SDS-10% PAGE gel, and stained with Coomassie brilliant blue. (B) Proteins in the gel were subjected to Western blot analysis using anti-SipA rabbit antiserum. Data are one of two similar results from two independent experiments.