FIG. 2.
SDS-PAGE analysis of proteins solubilized from mature spores. Spores were prepared at stage T18 of sporulation. Whole-protein samples were solubilized from the purified spores and analyzed by 14% (wt/vol) SDS-PAGE. Protein samples were prepared from the following strains: wild type (lane 1), yeeK mutant (lane 2), yabG mutant (lane 3), and yabG-yeeK double mutant (lane 4). Arrowheads indicate bands specifically found in yabG mutant spores. Asterisks indicate bands corresponding to the 18-kDa molecule of YeeK.