TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and/or relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| E. coli K-12 | ||
| MG1655 | F− λ−ilvG rfb-50 rph-1 | 40 |
| LVM100 (Δ5) | MG1655 ΔmazEF ΔrelBEF ΔchpB ΔyefM-yoeB ΔdinJ-yafQ | 40 |
| MG1655 fimA | K-12 MG1655 ΔfimA ΩKmr | This study |
| MG1655 yefM | K-12 MG1655 ΔyefM::Tn5Kan-2 | 16 |
| Δ5 fimA | Δ5 ΔfimA ΩKmr | This study |
| BW25113 | lacIqrrnBT14 ΔlacZWJ16hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | 1 |
| BW25113 fimA | K-12 BW25113 ΔfimA ΩKmr | 1 |
| BW25113 yjgK | K-12 BW25113 ΔyjgK ΩKmr | 1 |
| BW25113 mazF | K-12 BW25113 ΔmazF ΩKmr | 1 |
| BW25113 relE | K-12 BW25113 ΔrelE ΩKmr | 1 |
| BW25113 chpB | K-12 BW25113 ΔchpB ΩKmr | 1 |
| BW25113 yoeB | K-12 BW25113 ΔyoeB ΩKmr | 1 |
| BW25113 yafQ | K-12 BW25113 ΔyafQ ΩKmr | 1 |
| V. harveyi | ||
| BB170 | BB120 luxN::Tn5 (sensor 1−, sensor 2−) | 2 |
| Plasmids | ||
| pCA24N | Cmr; lacIq, pCA24N | 17 |
| pCA24N-yjgK | Cmr; lacIq, pCA24N PT5-lac::yjgK+ | 17 |
| pCA24N-relB | Cmr; lacIq, pCA24N PT5-lac::relB+ | 17 |
| pCA24N-dinJ | Cmr; lacIq, pCA24N PT5-lac::dinJ+ | 17 |
| pCA24N-yefM | Cmr; lacIq, pCA24N PT5-lac::yefM+ | 17 |
| pCA24N-mazF | Cmr; lacIq, pCA24N PT5-lac::mazF+ | 17 |
| pCA24N-relE | Cmr; lacIq, pCA24N PT5-lac::relE+ | 17 |
| pCA24N-chpB | Cmr; lacIq, pCA24N PT5-lac::chpB+ | 17 |
| pCA24N-yoeB | Cmr; lacIq, pCA24N PT5-lac::yoeB+ | 17 |
| pCA24N-yafQ | Cmr; lacIq, pCA24N PT5-lac::yafQ+ | 17 |
| pLW11 | Ampr; pFZY1 derivative containing plsrACDBFG::lacZ | 44 |
a
Kmr, Cmr, and Ampr are kanamycin, chloramphenicol, and ampicillin resistance, respectively.