TABLE 3.
Differentially expressed genes in biofilm cells of BW25113 yjgK versus the BW25113 wild-type strain in LB medium after incubation for 8 h at 37°C
| Group and gene | b no. | Fold change | Description |
|---|---|---|---|
| Small RNA related | |||
| ryeA | b4432 | -2.0 | Novel small RNA, function unknown |
| Small toxin related | |||
| hokA | b4455 | -1.5 | Small toxic membrane polypeptide |
| hokE | b4415 | -1.3 | Small toxic membrane polypeptide |
| Cell motility related | |||
| fimA | b4314 | 3.0 | Fimbrin type 1, major structural subunit; |
| fimB | b4312 | 1.7 | Recombinase involved in phase variation; regulator for fimA |
| fimI | b4315 | 2.5 | Required for pilus biosynthesis, FimA homolog |
| fimC | b4316 | 1.9 | Periplasmic chaperone, required for type 1 fimbriae |
| fimD | b4317 | 1.7 | Outer membrane protein; export and assembly of type 1 fimbriae |
| fimE | b4313 | 1.5 | Recombinase involved in phase variation; regulator for fimA |
| fimF | b4318 | 1.6 | Fimbrial morphology |
| fimG | b4319 | 1.7 | Fimbrial morphology |
| fimH | b4320 | 1.5 | Minor fimbrial subunit, d-mannose specific adhesin |
| hofB | b0107 | 1.9 | Putative integral membrane protein involved in biogenesis of fimbriae, protein transport, and DNA uptake |
| Iron transport related | |||
| fecA | b4291 | 1.9 | Outer membrane receptor; citrate-dependent iron transport |
| fecB | b4290 | 2.3 | Citrate-dependent iron transport, periplasmic protein |
| fecC | b4289 | 1.6 | Citrate-dependent iron(III) transport protein, cytosolic |
| fecD | b4288 | 1.6 | Citrate-dependent iron transport, membrane-bound protein |
| fecE | b4287 | 1.9 | ATP-binding component of citrate-dependent iron(III) transport protein |
| fecR | b4292 | 1.6 | Regulator for fec operon, periplasmic |
| fecI | b4393 | 1.5 | Probable RNA polymerase sigma factor |
| Cell division related | |||
| ftsL | b0083 | 1.6 | Cell division protein; ingrowth of wall at septum |
| ftsQ | b0093 | 1.5 | Cell division protein; ingrowth of wall at septum |
| ftsZ | b0095 | 1.5 | Cell division; forms circumferential ring; tubulinlike GTP-binding protein and GTPase |
| Hydrogenase related | |||
| hyaB | b0973 | -1.6 | Hydrogenase-1 large subunit |
| hyaC | b0974 | -1.4 | Probable NiFe-hydrogenase 1 b-type cytochrome subunit |
| hyaD | b0975 | -1.5 | Processing of HyaA and HyaB proteins |
| hyaE | b0976 | -1.5 | Processing of HyaA and HyaB proteins |
| hyaF | b0977 | -1.4 | Nickel incorporation into hydrogenase-1 proteins |