TABLE 1.
Distribution of blcC among Agrobacterium and Rhizobium isolates
| Straina | Biovarf | TraR-QS (reference)b | C58 blcC homolog(s)c | Growth ond:
|
|
|---|---|---|---|---|---|
| Glucose | GBL | ||||
| Agrobacterium spp. | |||||
| C58* | 1 | E, S (39) | SA+, G+ | +++++ | +++++ |
| A6* | 1 | E, S (59) | SA+ | +++++ | +++++ |
| 15955* | 1 | E, S (16) | SA+ | +++++ | +++ |
| R10 | 1 | E, S (18) | SA+ | +++++ | +++++ |
| Ach5 | 1 | E, S (59) | SA+ | +++++ | ++++ |
| IIBv7 | 1 | ND | SA+ | +++++ | ++++ |
| Chry5* | 1 | E, S (34) | SA+ | +++++ | ++ |
| Ant4* | 1 | E (51) | SA+ | +++++ | +++ |
| Bo542* | 1 | E, S (34) | SA− | +++++ | − |
| T37 | 1 | E (14) | SA− | +++++ | + |
| K599* | 1 | S (4) | SA− | +++++ | + |
| B6S3e | 1 | E, S (46) | SA− | +++++ | − |
| B6-Schile | 1 | E, S (46) | SA− | +++++ | − |
| K84* | 2 | E, S (35) | SA−, G− | +++++ | − |
| J73 | 2 | ND | SA− | +++++ | + |
| AB2-73* | 2 | E (50) | SA− | +++++ | − |
| A4* | 2 | E, S (36) | SA− | +++++ | − |
| K299* | 2 | E (35) | SA− | +++++ | + |
| 8196 | 2 | E (36) | SA− | +++++ | − |
| S4 | 3 | S | SA− | +++++ | − |
| TM4 | 3 | ND | SA− | +++++ | − |
| R. leguminosarum 3841 | NA | E, S (55) | G+ | +++++ | ++++ |
| R. etli CFN42 | NA | E, S (48) | G− | +++++ | + |
All listed strains are held in our collection. Strains marked with an asterisk have been reported to produce N-(3-oxooctanoyl)-l-homoserine lactone (4).
TraR-QS, presence of a plasmid coding for a TraR-type quorum-sensing system; E, experimentally determined; S, determined by sequence analysis; ND, no data available.
Determined by moderate-stringency Southern analysis using a blcC probe (SA+ and SA−) and by Blastn, Blastp, and TBlastn analyses of genome sequences available in public databases (G+ and G−).
Growth on solid minimal medium with the indicated substrate as the sole carbon source was assessed visually and graded from +++++ (luxuriant growth) to − (no detectable growth).
Two isolates of strain B6 with different plasmid complements (20).
NA, not applicable.