TABLE 1.
The mutagenic primers for hybrid parB genesa
| Plasmid | Par protein species | Mutation(s) | Mutagenic primer sequence |
|---|---|---|---|
| pALA2941 | P1 (P7) | R287, R288 | CTGAATTATGGAAATTTGAGGACCGTCGCCGCTTTGCAAGGAAGCGCGTG |
| pALA2944 | P1 (P7) | R287 | CTGAATTATGGAAATTTGAGGACCGTGATCGCTTTGCAAGGAAGCGCGTG |
| pALA2945 | P1 (P7) | R288 | GGAAATTTGAGGACAAGCGCCGCTTTGCAAGGAAGCG |
| pALA2946 | P1 (P7) | Q289 | GGAAATTTGAGGACAAGGATCAATTTGCAAGGAAGCGCGTGAAAGG |
| pALA2958 | P1 (P7) | D283 | CCGTAGTTACTGAATTATGGGACTTTGAGGACAAGGATCGC |
| pALA2959 | P1 (P7) | S285 | CTGAATTATGGAAATTTTCTGACAAGGATCGCTTTGC |
| pALA2960 | P1 (pMT1) | R287 | GAATTATGGAAATTTGAGGACCGTGATCGCTTTGCAAGGAAGCGC |
| pALA2935 | P1 (pMT1) | N288 | TATGGAAATTTGAGGACCGTAATCGCTTTGCAAGGAAGCGCGT |
| pALA2936 | P1 (pMT1) | Q289 | GGAAATTTGAGGACAAGGATCAGTTTGCAAGGAAGCGCGTGAAAG |
| pALA2923 | P1 (pMT1) | R287, N288 | GAATTATGGAAATTTGAGGACCGTAATCGCTTTGCAAGGAAGCGCGTG |
| pALA2961 | P7 (P1) | K283 | TCTGTGGTGGTTGAGAAGCTGCGAAAATTCTCTGACCGTCGCCAATATGCCC |
| pALA2962 | P7 (P1) | E285 | GGTTGAGAAGCTGCGAGACTTCGAGGACCGTCGCCAATATGCCCGAAAG |
| pALA2947 | P7 (P1) | K287 | GCTGCGAGACTTCTCTGACAAGCGCCAATATGCCCG |
| pALA2948 | P7 (P1) | D288 | GCGAGACTTCTCTGACCGTGATCAATATGCCCGAAAGAAG |
| pALA2949 | P7 (P1) | R289 | CTCTGACCGTCGCCGCTATGCCCGAAAGAAG |
| pALA2950 | P7 (P1) | K287D288 | GAGAAGCTGCGAGACTTCTCTGACAAGGATCAATATGCCCGAAAGAAGTCCG |
| pALA2937 | pMT1 (P1) | K287D288 | GAGAAGCTGAGAGAATTTTCAGATAAGGATCAGTTTGCCAGAAAGAAAACTG |
| pALA2939 | pMT1 (P1) | K287 | GAAGCTGAGAGAATTTTCAGATAAGAATCAGTTTGCCAGAAAGAAAAC |
a
The forward primers for site-specific mutagenesis are shown. The codons corresponding to the altered amino acids are underlined. The reverse primers were the complements of the forward primers. Each plasmid expresses a ParA protein and a ParB protein from the species indicated and has a 1- or 2-amino-acid substitution in ParB from the species shown in brackets.