TABLE 2.
Hybrid ParB proteins can switch to a new type of specificity in partition
| ParB protein starting sequence | Modification to ParB residues 287-288a (DRS) | % Retention of test plasmid with parS site from:
|
||
|---|---|---|---|---|
| P1 | P7 | pMT1 | ||
| P1 | None | 98 | <1 | <1 |
| P1⇒P7 (KD⇒RR) | <1 | 95 | <1 | |
| P1⇒pMT1 (KD⇒RN) | 93 | 20 | 90 | |
| P7 | None | <1 | 89 | <1 |
| P7⇒P1 (RR⇒KD) | 8 | <1 | <1 | |
| pMT1 | None | <1 | <1 | 94 |
| pMT1⇒P1 (RN⇒KD) | 9 | <1 | <1 | |
a
Numbering refers to the P1 ParB residues and their equivalents in the other ParB species according to the alignment shown in Fig. 1. The values shown represent the retention of the plasmid during 25 generations of unselected growth in a single experiment. Two repeat experiments gave similar values.