TABLE 3.
ParB recognition specificity can be changed by a single-amino-acid substitution
| Original ParB species | Substitutiona | % Retention of test plasmidb with parS site from:
|
||
|---|---|---|---|---|
| P1 | P7 | pMT1 | ||
| P1 | K283D (P7) | 94 | <1 | ND |
| E285S (P7) | 90 | <1 | ND | |
| K287R (P7) | 98 | <1 | ND | |
| D288R (P7) | <1 | 71 | ND | |
| R289Q (P7) | 93 | <1 | ND | |
| K287R (pMT1) | 70 | ND | <1 | |
| D288N (pMT1) | 83 | ND | 90 | |
| R289Q (pMT1) | 98 | ND | <1 | |
| P7 | D283K (P1) | <1 | 90 | ND |
| S285E (P1) | <1 | 94 | ND | |
| R287K (P1) | <1 | 97 | ND | |
| R288D (P1) | 13c | <1d | ND | |
| Q289R (P1) | >1 | 96 | ND | |
a
Numbering is as described for Table 2. Letters refer to amino acids before and after the change, and the source of the novel amino acid is given in parentheses.
b
Retention was measured during 25 generations of unselected growth. ND, not determined.
c
In four repeat experiments, the mean value with P1 parS was 12.8, with a standard deviation of 3.
d
The values for P7 parS were less than 1 in each of these repeat experiments.