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l-Alanine-activated germination of spores produced by the wild-type and Δalr strains of B. anthracis. Wild-type (A) and Δalr (B) spores were incubated in the presence of the different concentrations of l-alanine indicated, and germination was monitored by determining the decrease in the OD580 at selected time points. A 70% decrease in the OD580 corresponded to germination of essentially all spores in the sample. WT, wild type.
