TABLE 1.
Strains used in this studya
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| B. subtilis | ||
| PY79 | Prototroph, SPβ0 | 89 |
| LAS24 | recA::neo | 75 |
| LAS40 | recA-mgfp-mut2(A206K) (spc) | 78 |
| LAS167 | recN::cat | 70 |
| LAS195 | amyE::PxylI-SceI (cat) | 78 |
| LAS206 | xkdF-yfp (spc) | 15; this study |
| LAS210 | amyE::PxylI-SceI (mls), cgeD::I-SceI recognition site (cat) | 78 |
| LAS239 | amyE::PxylI-SceI (mls), cgeD::I-SceI recognition site (cat), xkdF-yfp (spc) | This study |
| LAS261 | amyE::PxylI-SceI (mls), cgeD::I-SceI recognition site (cat), recA-mgfp(A206K) (spc) | 78 |
| LAS320 | ykoU-ykoV::erm | 85; this study |
| LAS366 | tagC-gfp (spc) | 15; this study |
| LAS367 | amyE::PxylI-SceI (mls) cgeD::I-SceI recognition site (cat), tagC-gfp (spc) | This study |
| LAS600 | trpC2 Δupp | 30 |
| LAS630 | trpC2 Δupp recA::neo | This study |
| LAS631 | trpC2 Δupp dinR3 [lexA(Ind−)] | This study |
| LAS634 | tagC-gfp (spc) recO::cat | This study |
| LAS635 | tagC-gfp (spc) recN::cat | This study |
| E. coli | ||
| MC4100 | F−araD139 Δ(argF-lac)U169 relA1 rpsL150(Strr) flbB5301 deoC1 ptsF25 rbsR | Laboratory stock |
| SS996 | Δattλ::sulApΩgfp-mut2 | 51 |
| BWD06 | MC4100 recA4136-gfp | 64; this study |
| BWD11 | MC4100 ΔrecA::Tc | This study |
| BWD12 | MC4100 lexA3 [lexA(Ind−)] malF::kan | This study |
a
All B. subtilis strains used in this study are derivatives of 168. All BWD derivatives were constructed from MC4100.