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. 2008 Dec 1;77(2):877–884. doi: 10.1128/IAI.01017-08

FIG. 5.

FIG. 5.

Immunization efficacy and safety of B. abortus S19 ΔvjbR::Kan vaccine formulations. BALB/c mice were immunized i.p. with 1 × 105 of either nonencapsulated or encapsulated S19 ΔvjbR::Kan. Control groups received empty capsules or S19. After 20 weeks, the mice were challenged i.p. with 1 × 105 CFU wild-type 2308. At 1 week postchallenge, the mice were euthanized, their spleens harvested, and the bacterial loads (A) and spleen weights (B) determined. (A) Values are reported as the mean log10 recovery of the 2308 challenge organism recovered from the spleens. Differences in colonization between all the groups were determined by ANOVA followed by a Tukey's posttest (*, P < 0.05; **, P < 0.001). (B) Spleen weights were measured in mg and were compared and analyzed by ANOVA followed by a Tukey's posttest comparing all groups to one another (*, P < 0.01; **, P < 0.001). For the statistical representation on both graphs, “a” symbolizes naïve animals, “b” S19-vaccinated animals, “c” encapsulated S19 ΔvjbR::Kan-vaccinated animals, and “d” nonencapsulated S19 ΔvjbR::Kan-vaccinated animals.