FIG. 6.
IgG1 and IgG2 anti-Brucella antibodies in serum from mice immunized with S19 ΔvjbR::Kan. BALB/c mice were inoculated i.p. with 1 × 105 CFU of either nonencapsulated S19 ΔvjbR::Kan or encapsulated S19 ΔvjbR::Kan. Mice within the control group received empty capsules in lieu of vaccine. At 0, 3, 7, and 21 weeks postvaccination (1 week postchallenge), serum samples were collected for IgG1 (A) and IgG2 (B) determination by ELISA. Results are shown as the means ± SEM of absorbance at 450 nm. For the statistical representation on both graphs, “a” symbolizes naïve animals, “b” encapsulated S19 ΔvjbR::Kan-vaccinated animals, and “c” nonencapsulated S19 ΔvjbR::Kan-vaccinated animals.