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. 2008 Dec 8;77(2):749–755. doi: 10.1128/IAI.00764-08

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FIG. 4. — Detection of PA, LF, and γ-DPGA complexes in fractionated rabbit and guinea pig plasma. Infected rabbit and guinea pig plasma was fractionated by size exclusion column chromatography, and fractions were analyzed by PA capture ELISA. In the rabbit (A), PA was associated with γ-DPGA in fractions 6 to 10 and with LF in fractions 6 to 10 and LF protease activity was detected in fractions 7 to 11. In the guinea pig (B), PA was associated with γ-DPGA in fractions 6 to 12 and LF protease activity was detected in fractions 9 to 11. SDS-PAGE and native PAGE showed results similar to those in Fig. 2A through D (not shown). Pure recombinant PA and LF eluted at fraction 16 (not shown). In vitro-produced LT (PA63 plus LF) eluted at fraction 13 (not shown). RFU, relative fluorescence units; Abs, absorbance.