TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| E. coli DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk− mk+) phoA supE44 λ−thi-1 gyrA96 relA1 | Invitrogen |
| S. enterica serovar Enteritidis strains | ||
| SE2472 | Clinical isolate | 32 |
| ΔtrkA | SE2472 trkA::kan | This study |
| ΔtrkA-comp | SE2472 trkA::kan transformed with pRB3-trkA | This study |
| SipA(HF) | SE2472 sipA::His-FLAG, SipA+ | This study |
| SipC(HF) | SE2472 sipC::His-FLAG, SipC+ | This study |
| SopB(HF) | SE2472 sopB::His-FLAG, SopB+ | This study |
| SipA(HF)ΔtrkA | SipA(HF) trkA::kan | This study |
| SipC(HF)ΔtrkA | SipC(HF) trkA::kan | This study |
| SopB(HF)ΔtrkA | SopB(HF) trkA::kan | This study |
| SipA(HF)ΔtrkA-comp | SipA(HF)ΔtrkA transformed with pRB3-trkA | This study |
| SipC(HF)ΔtrkA-comp | SipC(HF)ΔtrkA transformed with pRB3-trkA | This study |
| SopB(HF)ΔtrkA-comp | SopB(HF)ΔtrkA transformed with pRB3-trkA | This study |
| Plasmids | ||
| pUC-H1PF1 | Apr, derivative of pUC19 containing Kanr, FLAG, and His6 | This study |
| pKD4 | Apr Kanr oriRγ | 12 |
| pKD46 | Apr, contains the Red recombinase of λ phage | 12 |
| pCP20 | Aprcat cl857 λPr flp pSC101 oriTS | 12 |
| pRB3-273C | Apr, low- to medium-copy-number plasmid for Salmonella | 12 |
| pRB3-trkA | Derivative of pRB3-273C containing trkA | This study |