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. 2008 Dec 1;77(2):725–732. doi: 10.1128/IAI.01305-08

FIG. 4.

FIG. 4.

Effect of complement and CD14 inhibition on wCD11R3 expression on granulocytes. (A) Different amounts of E. coli were added to porcine whole blood (n = 2) and incubated for 10 min at 37°C. The samples were analyzed for wCD11R3 expression using a FACScan flow cytometer. The means and ranges of two separate experiments are shown. MFI, median fluorescence intensity. (B) Gating of the granulocytes as shown by a forward scatter-side scatter plot. (C) Histogram showing the shift of the fluorescence intensity (FI) from 105 E. coli bacteria/ml (filled area) or PBS alone to 108 E. coli bacteria/ml. (D) Whole blood from two pigs was preincubated for 5 min with VCP, anti-CD14, or HSA as a control. A fixed dose of 108 E. coli bacteria/ml whole blood was then added and incubated for 10 min at 37°C. The samples were analyzed to determine wCD11R3 expression using a FACScan flow cytometer. The results of one of two virtually identical experiments are shown. (E) Gating of the granulocytes, as shown in a forward scatter-side scatter plot. (F) Histogram showing the shift of fluorescence intensity from 108 E. coli cells/ml with PBS (filled area), with HSA, and with anti-CD14 to VCP. The results for an irrelevant detection control antibody (Irr. control Ab) are also indicated.