FIG. 3.
Cell surface expression and trimer formation of truncated DsrA proteins. (A) Sarkosyl-insoluble OMPs (approximately 1 μg per lane) were solubilized at 100°C (panel 1) and 37°C (panel 2) and subjected to Western blotting with anti-rFL-DsrAI. (B) (Top panel) Whole-cell binding assay with anti-rNt-DsrAI, a rabbit polyclonal antibody that binds to the surface of H. ducreyi. (Bottom panel) Total cellular proteins from the indicated strains were solubilized at 100°C and subjected to Western blotting with anti-rNt-DsrAI. RLU, relative light units.