TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or phenotype | Protein expressed | Reference(s) |
|---|---|---|---|
| H. ducreyi strains | |||
| 35000HP | Wild-type, human-passaged variant of strain 35000 | DsrAI | 3, 16 |
| FX517 | 35000HPdsrA::CATa | None | 13 |
| Plasmids | |||
| pLSSK | H. ducreyi shuttle vector; Smr | None | 39 |
| pUNCH1260 | Complete dsrA ORF in pLSSK (774 bp; 257 amino acids) | DsrAI | 13 |
| pUNCH1424 | Base pairs 73 to 510 deleted in dsrA (corresponding to amino acids 25 to 170)b | DsrAIΔ25-170 | This study |
| pUNCH1425 | Base pairs 73 to 474 deleted in dsrA (corresponding to amino acids 25 to 158) | DsrAIΔ25-158 | This study |
| pUNCH1426 | Base pairs 73 to 438 deleted in dsrA (corresponding to amino acids 25 to 146) | DsrAIΔ25-146 | This study |
| pUNCH1427 | Base pairs 73 to 399 deleted in dsrA (corresponding to amino acids 25 to 133) | DsrAIΔ25-133 | This study |
| pUNCH1428 | Base pairs 73 to 363 deleted in dsrA (corresponding to amino acids 25 to 121) | DsrAIΔ25-121 | This study |
| pUNCH1429 | Base pairs 73 to 330 deleted in dsrA (corresponding to amino acids 25 to 110) | DsrAIΔ25-110 | This study |
| pUNCH1430 | Base pairs 73 to 291 deleted in dsrA (corresponding to amino acids 25 to 97) | DsrAIΔ25-97 | This study |
| pUNCH1431 | Base pairs 73 to 195 deleted in dsrA (corresponding to amino acids 25 to 65) | DsrAIΔ25-65 | This study |
a
CAT, chloramphenicol acetyl transferase cassette.
b
The nucleotide positions are the positions in the dsrA ORF of H. ducreyi strain 35000 (GenBank accession no. AF187001).