TABLE 2.
Oligonucleotides used in this study
| Primer | Description | Sequence (5′→3′)a |
|---|---|---|
| DsrA121s | Reverse primer used with DsrA611s, DsrA3776F, and DsrA3737F to create pUNCH1424, pUNCH1425, and pUNCH1426, respectively | TTTCCCGGGCTGCTGAGCCATTGTTGTAAT |
| DsrA611s | Forward primer used with DsrA121s to create pUNCH1424 | ATGCCCGGGCAAAATACACATAATATCAATAAG |
| DsrA3776F | Forward primer used with DsrA121s to create pUNCH1425 | CTAGAACCCGGGACTTATTTAGATG |
| DsrA3737F | Forward primer used with DsrA121s to create pUNCH1426 | AAAAATCCCGGGAATATTGATACTATAAGTAAATAT |
| DsrAxE | Reverse primer used with DsrAxG, DsrAxF, DsrAxD, DsrAxC, and DsrAxB to create pUNCH1427, pUNCH1428, pUNCH1429, pUNCH1430, and pUNCH1431, respectively | TTGCCCGGGCATACGATAAGAATCATCTAAATA |
| DsrAxB | Forward primer used with DsrAxE to create pUNCH1431 | AAGCCCGGGGAATGGATTTCTAAACAG |
| DsrAxC | Forward primer used with DsrAxE to create pUNCH1430 | TCTCCCGGGCCTATACTGTTATATCCGATGTC |
| DsrAxD | Forward primer used with DsrAxE to create pUNCH1429 | CAACCCGGGTAATCGGCAGCAG |
| DsrAxF | Forward primer used with DsrAxE to create pUNCH1428 | CTGAAATTGCCCGGGTATAGTTATTTTAACGATTTAAGAC |
| DsrAxG | Forward primer used with DsrAxE to create pUNCH1427 | AGACACGATCCCGGGTTAAAAGTTTCTGATGCAC |
| T3 | Sequencing primer | ATTAACCCTCACTAAAGGGA |
| T7 | Sequencing primer | TAATACGACTCACTATAGGG |
a
Underlining indicates the XmaI site.