Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1985 Feb;47(2):527–533. doi: 10.1128/iai.47.2.527-533.1985

Immunity and protection of mice against Neisseria meningitidis group B by vaccination, using polysaccharide complexed with outer membrane proteins: a comparison with purified B polysaccharide.

C Moreno, M R Lifely, J Esdaile
PMCID: PMC263203  PMID: 3917979

Abstract

A methodology for preparing outer membrane proteins (type specific) complexed to group B polysaccharide of Neisseria meningitidis is described. These complexes, low in nucleic acid and lipopolysaccharide content, were immunogenic in mice with induction of humoral antigroup B and antiprotein responses. Immunized mice were also protected against challenge with N. meningitidis group B strains of the same or a different type from that used for vaccination. Both immunity and protection were enhanced when the mice received a secondary immunization with the protein-polysaccharide complex. Additional data have shown the capacity of purified B polysaccharide to induce immunological memory, even though it is incapable of inducing a humoral response when given alone.

Full text

PDF

Images in this article

528Image
on p.528

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker P. J., Stashak P. W., Amsbaugh D. F., Prescott B. Characterization of the antibody response to type 3 pneumococcal polysaccharide at the cellular level. I. Dose-response studies and the effect of prior immunization on the magnitude of the antibody response. Immunology. 1971 Apr;20(4):469–480. [PMC free article] [PubMed] [Google Scholar]
  2. Beuvery E. C., Miedema F., van Delft R. W., Haverkamp J., Leussink A. B., te Pas B. J., Teppema K. S., Tiesjema R. H. Preparation and physicochemical and immunological characterization of polysaccharide-outer membrane protein complexes of Neisseria meningitidis. Infect Immun. 1983 Apr;40(1):369–380. doi: 10.1128/iai.40.1.369-380.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Borel Y., Reinisch C. L., Schlossman S. F. T and B cell in hapten-specific carrier-determined tolerance. J Exp Med. 1975 Nov 1;142(5):1254–1262. doi: 10.1084/jem.142.5.1254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brandt B. L., Wyle F. A., Artenstein M. S. A radioactive antigen-binding assay for Neisseria meningitidis polysaccharide antibody. J Immunol. 1972 Apr;108(4):913–920. [PubMed] [Google Scholar]
  5. Del Guercio P. Effect of adjuvants on the antibody response to a hapten on a thymus-independent carrier. Nat New Biol. 1972 Aug 16;238(85):213–215. doi: 10.1038/newbio238213a0. [DOI] [PubMed] [Google Scholar]
  6. Finne J., Leinonen M., Mäkelä P. H. Antigenic similarities between brain components and bacteria causing meningitis. Implications for vaccine development and pathogenesis. Lancet. 1983 Aug 13;2(8346):355–357. doi: 10.1016/s0140-6736(83)90340-9. [DOI] [PubMed] [Google Scholar]
  7. Finne J. Occurrence of unique polysialosyl carbohydrate units in glycoproteins of developing brain. J Biol Chem. 1982 Oct 25;257(20):11966–11970. [PubMed] [Google Scholar]
  8. Frasch C. E., Robbins J. D. Protection against group B meningococcal disease. II. Infection and resulting immunity in a guinea pig model. J Exp Med. 1978 Mar 1;147(3):619–628. doi: 10.1084/jem.147.3.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gotschlich E. C., Austrian R., Cvjetanović B., Robbins J. B. Prospects for the prevention of bacterial meningitis with polysaccharide vaccines. Bull World Health Organ. 1978;56(4):509–518. [PMC free article] [PubMed] [Google Scholar]
  10. Gotschlich E. C., Fraser B. A., Nishimura O., Robbins J. B., Liu T. Y. Lipid on capsular polysaccharides of gram-negative bacteria. J Biol Chem. 1981 Sep 10;256(17):8915–8921. [PubMed] [Google Scholar]
  11. Gotschlich E. C., Goldschneider I., Artenstein M. S. Human immunity to the meningococcus. IV. Immunogenicity of group A and group C meningococcal polysaccharides in human volunteers. J Exp Med. 1969 Jun 1;129(6):1367–1384. doi: 10.1084/jem.129.6.1367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gotschlich E. C., Liu T. Y., Artenstein M. S. Human immunity to the meningococcus. 3. Preparation and immunochemical properties of the group A, group B, and group C meningococcal polysaccharides. J Exp Med. 1969 Jun 1;129(6):1349–1365. doi: 10.1084/jem.129.6.1349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gotschlich E. C., Rey M., Etienne J., Sanborn W. R., Triau R., Cvjetanović B. The immunological responses observed in field studies in Africa with group A meningococcal vaccines. Prog Immunobiol Stand. 1971;5:485–491. [PubMed] [Google Scholar]
  14. Hoffman S., Edelman G. M. Kinetics of homophilic binding by embryonic and adult forms of the neural cell adhesion molecule. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5762–5766. doi: 10.1073/pnas.80.18.5762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hosokawa T. Studies on B-cell memory. II. T-cell independent antigen can induce B-cell memory. Immunology. 1979 Oct;38(2):291–299. [PMC free article] [PubMed] [Google Scholar]
  16. Howard J. G., Christie G. H., Courtenay B. M. Studies on immunological paralysis. IV. The relative contributions of continuous antibody neutralization and central inhibition to paralysis with type 3 pneumococcal polysaccharide. Proc R Soc Lond B Biol Sci. 1971 Sep 28;178(1053):417–438. doi: 10.1098/rspb.1971.0073. [DOI] [PubMed] [Google Scholar]
  17. Jennings H. J., Lugowski C. Immunochemistry of groups A, B, and C meningococcal polysaccharide-tetanus toxoid conjugates. J Immunol. 1981 Sep;127(3):1011–1018. [PubMed] [Google Scholar]
  18. Klaus G. G., Humphrey J. H. B cell tolerance induced by polymeric antigens. I. Comparison of the dose and epitope density requirements for inactivation of primed and unprimed B cells in vivo. Eur J Immunol. 1976 Jun;5(6):361–365. doi: 10.1002/eji.1830050602. [DOI] [PubMed] [Google Scholar]
  19. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  20. Lifely M. R., Gilbert A. S., Moreno C. Rate, mechanism, and immunochemical studies of lactonisation in serogroup B and C polysaccharides of Neisseria meningitidis. Carbohydr Res. 1984 Dec 1;134(2):229–243. doi: 10.1016/0008-6215(84)85040-5. [DOI] [PubMed] [Google Scholar]
  21. Lifely M. R., Gilbert A. S., Moreno C. Sialic acid polysaccharide antigens of Neisseria meningitidis and Escherichia coli: esterification between adjacent residues. Carbohydr Res. 1981 Aug 1;94(2):193–203. doi: 10.1016/s0008-6215(00)80717-x. [DOI] [PubMed] [Google Scholar]
  22. Lindon J. C., Vinter J. G., Lifely M. R., Moreno C. Conformational and dynamic differences between N. meningitidis serogroup B and C polysaccharides, using n.m.r. spectroscopy and molecular mechanics calculations. Carbohydr Res. 1984 Oct 1;133(1):59–74. doi: 10.1016/0008-6215(84)85183-6. [DOI] [PubMed] [Google Scholar]
  23. Mandrell R. E., Zollinger W. D. Measurement of antibodies to meningococcal group B polysaccharide: low avidity binding and equilibrium binding constants. J Immunol. 1982 Nov;129(5):2172–2178. [PubMed] [Google Scholar]
  24. Moreno C., Esdaile J. Immunoglobulin isotype in the murine response to polysaccharide antigens. Eur J Immunol. 1983 Mar;13(3):262–264. doi: 10.1002/eji.1830130317. [DOI] [PubMed] [Google Scholar]
  25. Moreno C., Hewitt J., Hastings K., Brown D. Immunological properties of monoclonal antibodies specific for meningococcal polysaccharides: the protective capacity of IgM antibodies specific for polysaccharide group B. J Gen Microbiol. 1983 Aug;129(8):2451–2456. doi: 10.1099/00221287-129-8-2451. [DOI] [PubMed] [Google Scholar]
  26. Peppler M. S., Frasch C. E. Protection against group B Neisseria meningitidis disease: effect of serogroup B polysaccharide and polymyxin B on immunogenicity of serotype protein preparations. Infect Immun. 1982 Jul;37(1):264–270. doi: 10.1128/iai.37.1.264-270.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. SVENNERHOLM L. Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta. 1957 Jun;24(3):604–611. doi: 10.1016/0006-3002(57)90254-8. [DOI] [PubMed] [Google Scholar]
  28. Tiesjema R. H., Beuvery E. C., te Pas B. J. Enhanced stability of meningococcal polysaccharide vaccines by using lactose as a menstruum for lyophilization. Bull World Health Organ. 1977;55(1):43–48. [PMC free article] [PubMed] [Google Scholar]
  29. Wyle F. A., Artenstein M. S., Brandt B. L., Tramont E. C., Kasper D. L., Altieri P. L., Berman S. L., Lowenthal J. P. Immunologic response of man to group B meningococcal polysaccharide vaccines. J Infect Dis. 1972 Nov;126(5):514–521. doi: 10.1093/infdis/126.5.514. [DOI] [PubMed] [Google Scholar]
  30. Zollinger W. D., Mandrell R. E., Altieri P., Berman S., Lowenthal J., Artenstein M. S. Safety and immunogenicity of a Neisseria meningitidis type 2 protein vaccine in animals and humans. J Infect Dis. 1978 Jun;137(6):728–739. doi: 10.1093/infdis/137.6.728. [DOI] [PubMed] [Google Scholar]
  31. Zollinger W. D., Mandrell R. E., Griffiss J. M., Altieri P., Berman S. Complex of meningococcal group B polysaccharide and type 2 outer membrane protein immunogenic in man. J Clin Invest. 1979 May;63(5):836–848. doi: 10.1172/JCI109383. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES