FIG. 1.

Species specificity of binding of fH to N. meningitidis. (A) Binding of goat polyclonal anti-human fH to fH in primate sera (1:100 dilution) as measured by Western blot analysis. The antibody cross-reacts with fH in each of the primate sera. MW, molecular mass marker. (B) Binding of fH to N. meningitidis strain H44/76 after incubation of bacteria in different primate sera. After being washed, the pelleted bacteria were lysed in sample buffer, the lysate was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and binding of fH was detected by Western blot analysis using the antisera described for panel A.