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. 2008 Dec 1;77(2):764–769. doi: 10.1128/IAI.01191-08

FIG. 2.

FIG. 2.

Regulation of rat C3 binding by human fH. Live bacteria were incubated with 20% rat sera. Rat C3 deposition was measured by flow cytometric detection as described in Materials and Methods. (A) Group B strains H44/76 and NZ98/254. Dotted histograms, control (no sera); open histograms (solid line), rat sera alone with 0 μg/ml of human fH; shaded histograms, rat sera plus human fH, 25 μg/ml. (B) Group B strain H44/76. Dotted histograms, control (no sera); shaded histograms, rat sera plus different concentrations of human fH incubated with bacteria (left) or, as a control, zyomsan (right), which does not bind fH.