FIG. 6.
Neutrophils are required for anti-BcfA antibody-mediated clearance of B. bronchiseptica. Mice were intraperitoneally injected with 1 mg of RB6-8C5 antibody or PBS. One day later, mice were intraperitoneally injected with 200 μl of anti-BcfA serum or naïve rat serum, followed by intranasal challenge with 5 × 105 CFU of RB50 in a 25-μl volume. Mice were sacrificed 2 days postchallenge, and bacterial colonization in the lungs was determined. Dashed line represents lower limits of CFU detection. Black bars represent mean colonization of respective group. The unpaired two-tailed Student t test was used to determine statistical significance. Asterisks indicate a P value of ≤ 0.05.