FIG. 7.

BcfA-induced production of IFN-γ and IL-4 in splenocytes. Mice were immunized at 0 and 3 weeks with either 30 μg of BcfA adsorbed to alum or 50 μg of alum only. Two and 4 weeks postimmunization, splenocytes were harvested and restimulated with 10 μg of purified BcfA protein. Supernatants were harvested 3 days poststimulation and analyzed for IFN-γ and IL-4 production using respective ELISA kits. Restimulations were carried out in triplicate for each individual mouse, and means ± standard deviations of individual mice are represented. The unpaired two-tailed Student t test was performed to determine statistical significance. Asterisks indicate a P value of ≤ 0.0005.