TABLE 2.

BcfA immunization reduces lung pathology^a

Pathology parameters	Total pathology scores (±SD) for mice treated with:
	10 μg BcfA	30 μg BcfA	Alum
Consolidation	0	0.27 ± 0.5	1.6 ± 1.1
Alveolar walls	1.7 ± 0.5	1.5 ± 0.5	1.8 ± 0.5
Degeneration	0	0.18 ± 0.4	1 ± 0.7
Edema	0.4 ± 0.5	0	1.2 ± 0.8
Hemorrhage	0.1 ± 0.4	0.09 ± 0.3	0.6 ± 0.9
Alveolar/interstitial PMNs	0.7 ± 0.5	0.3 ± 0.5	2 ± 1.0
Intrabronchial PMNs	0	0.09 ± 0.3	1 ± 0.7
Perivascular/peribronchiolar lymphocytes	0	0.09 ± 0.3	0.4 ± 0.6
Alveolar macrophages	0	0.2 ± 0.4	0.4 ± 0.6
Average total score	3 ± 1.2*	2.7 ± 2**	10 ± 5.4

^aMice were immunized with 10 or 30 μg of BcfA adsorbed to alum or alum only and challenged with RB50 as described in the legend to Fig. 1. Six days postchallenge, mice were sacrificed and the right lungs were harvested and processed for hematoxylin and eosin staining. The sections were examined by N. Kock in a manner that was blind to the treatment groups. The unpaired two-tailed Student t test was used to compare pathology scores between BcfA-immunized and alum-immunized mice. *, P ≤ 0.05; **, P ≤ 0.005; PMN, polymorphonuclear leukocyte.