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. 2008 Dec 15;77(2):604–614. doi: 10.1128/IAI.00907-08

FIG. 4.

FIG. 4.

IL-1R−/− mice have C. rodentium loads and neutrophil responses equivalent to those seen in C57BL/6 mice. (A) Various tissues were harvested aseptically from C57BL/6 (n = 12) and IL-1R−/− (n = 9) mice 7 days p.i. C. rodentium CFU were quantitated as described in Materials and Methods. MLN, mesenteric lymph nodes. (B) Pathology scores for recruitment measurements, including edema and polymorphonuclear leukocyte infiltration (PMN Infil.), were assessed in colonic tissue from C57BL/6 (n = 12) or IL-1R−/− (n = 13) mice 7 days p.i. (*, statistical significance in comparison to C57BL/6 samples, P < 0.01). (C) Manual neutrophil counts in crypts from C57BL/6 (n = 5 to 15) and IL-1R−/− (n = 7 to 15) colons (*, statistical significance in comparison to uninfected [uninf] C57BL/6 samples, P < 0.01; **, statistical significance in comparison to uninfected IL-1R−/− samples, P < 0.02; #, statistical significance in comparison to uninfected samples, P < 0.001). (D) CFU (5 × 105) of C. rodentium incubated with neutrophils (2.5 × 105) from C57BL/6 or IL-1R−/− mice were killed with equal efficacies. Results are from a representative experiment repeated three times in triplicate. Error bars represent the standard deviations.