TABLE 1.

qPCR primers and probes

Gene	Primer/probe	Sequence (5′-3′)	GenBank accession no.
ARP	ARP 67 Fwd	CTG CAA AGA TGC CCA GGG A	NM_131580
	ARP 187 Rev	TTG GAG CCG ACA TTG TCT GC
	ARP Probe	TTC TGA AAA TCA TCC AAC TGC TGG ATG ACT ACCa
IFN-γ	IFN-γ Fwd	CTT TCC AGG CAA GAG TGC AGA	NM_212864
	IFN-γ Rev	TCA GCT CAA ACA AAG CCT TTC G
	IFN-γ Probe	ACC GCT ATG GGC GAT CAA GGA AAA CGA C
TNF-α	TNF-α Fwd	TCG CAT TTC ACA AGG CAA TTT	NM_212859
	TNF-α Rev	GGC CTG GTC CTG GTC ATC TC
	TNF-α Probe	AGG CTG CCA TCC ATT TAA CAG GTG GAT ACA A
IL-1β	IL-1β Fwd	CAT CCA AGA GCG TGA AGT GAA	NM_212844
	IL-1β Rev	AAC ACA CAG GCT GAG CAG AAG T
	IL-1β Probe	TCC ACA TTT GCA GGC CGT CAC C
iNOS	iNOS Fwd	CCA GAG CCT TCG TCT GGA GA	NM_001104937
	iNOS Rev	TTA GAG CCT GGA CGA GCG TG
	iNOS Probe	CCA GCC ATG AGG GAA AGA GAT GGC AG
Francisella sp. 16S rRNA	FspQPCR Fwd	GCG AAG GCA ACA TTC TGG AT	DQ377177
	FspQPCR Rev	GCG TTG CAT CGA ATT AAA CCA
	FspQPCR probe	AAT ACT GAC ACT GAG GGA CGA AAG CGT GG

^aThere is a single-nucleotide polymorphism between the sequence for the EKW fish used in our experiments and the sequence in GenBank. The nucleotide is in bold; in the GenBank sequence, the A is a T.