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. 2008 Dec 1;77(2):810–816. doi: 10.1128/IAI.01293-08

TABLE 3.

Virulence of mutants in hamster model of acute infection

Straina Location of insertion Predicted function of mutated gene or descriptionb Locationc L. biflexad Dose(s)e Hamster survivalf
L495 Wild-type control 103 0/5
EMJH Negative control 5/5
M739 LB125 Chemotaxis-related protein Unknown Y 103, 105 0/4
M775* LB328 OmpA family protein OM N 103, 105 2/8
M776 LA1857 Fur paralogue C Y 103, 105 0/8
M777 intergenic Intergenic mutant control 103, 105 0/4
M780 LA2469 CheX, inhibitor of MCP methylation C Y 103, 105 0/4
M789 LA3028 HP, contains leucine-rich repeats Unknown N 103, 105 0/4
M894 LA3258 TonB-dependent receptor OM Y 103, 105 0/4
M880* LA4161 Metalloprotease Secreted N 103, 105 0/4
M834 LA3881 HP OM N 103, 105 0/4
M895# LA1641 HP IM N 103, 105 4/4
M1061* LA0709 HP IM N 103 1/5
M765* LA3097 LipL71 OM Y 103 0/5
M1224* LA1499 HP OM N 103 0/5
M886* LA2003 HP OM N 103 0/5
M1086* LA4129 HP, ankyrin repeat protein Unknown N 103 0/5
M874# LA0615 HP Unknown N 103 5/5
M421 LA4324 LenE OM N 103 1/5
M911 LA4209 HP Unknown N 104 0/5
M977 LA3103 LenB Unknown N 104 0/5
M983* LB362 HP Unknown N 103 0/5
M1065 LA1252 CheB, chemotaxis response regulator C Y 104 2/5
M1115* LA0676 MCP IM Y 105 0/5
M1225* LA0136 HP, LipL45-related protein Unknown Y 103 0/5
M1014* LA2215 Flagellar motor protein/OmpA family protein Unknown Y 103 2/5
M1073* LA0137 HP Unknown N 103 1/5
M968 LA3075 LigC Unknown N 103 0/5
M1020* LA0505 HP Unknown Y 103 0/5
M1059* LA4122 RNA polymerase sigma subunit C Y 103 0/5
M1229* LA3367 HP Unknown N 103 0/5
a

Mutants were constructed in L. interrogans serovar Manilae L495. *, the genotype of reisolates was confirmed by PCR; #, bacteria could not be recovered from animals.

b

Predicted function of protein encoded by disrupted gene. HP, hypothetical protein; MCP, methyl-accepting chemotaxis protein.

c

Predicted subcellular location of disrupted gene product using the psortb software program (www.psort.org/psortb/); C, cytoplasmic; OM, outer membrane; IM, inner membrane.

d

Indicates the presence of a deduced protein in L. biflexa with >50% similarity by BLASTP. Y, yes; N, no.

e

Mutants were injected intraperitoneally in two doses (103 and 105 leptospires) into groups of two or four hamsters, or a single dose was injected into groups of five hamsters.

f

No. surviving/total. Survival data are pooled for both doses if appropriate.