TABLE 2.

PCR primers used in this study

Primer name	Sequence (5′-3′)
Primers for PCR amplification of NBU1-joined ends and sigma 70
Sigma 70 Fwd	AGGACCTGATTACAGTCGAGGAAGAAGTGG
Sigma 70 Rev	ATTTCCCTCGCAAGAGACTCATTAACCAGA
NBU1-LJ	GGTAACTTATTTACTACGTTCA
NBU1-RJ	GATTGTAGCCATGGATTCTGT
Primers used for real-time RT-PCR of sigma 70 and rteB
Sigma 70 FP	ACGCTGTATGGTGGATTCGTCAGT
Sigma 70 RP	ACCTGATTCAACGGGAGACGAACA
F-rteB-RT	TGGTAGCCGCCACCGACATGAG
R-rteB-RT	CCGCAGTCCACCGCCACAAATG
Primers used to amplify rteB for pRteB
F-RteB-D55E-NdeI	ATTGCTTAATCCATATGGATAAGACA
R-D55E-HindIII-RteB	CAAATTCAGTGAAGCTTGGGCTATGATTGC
Primers used for site-directed mutagenesis of rteB
RteB-D55E-F	ATCGTGGTTGCCGAACTGCGTCTGCCTGAC
GC-RteB-D55E-F	GTCAGGCAGACGCAGTTCGGCAACCACGAT