Primers for PCR amplification of NBU1-joined ends and sigma 70 |
|
Sigma 70 Fwd |
AGGACCTGATTACAGTCGAGGAAGAAGTGG |
Sigma 70 Rev |
ATTTCCCTCGCAAGAGACTCATTAACCAGA |
NBU1-LJ |
GGTAACTTATTTACTACGTTCA |
NBU1-RJ |
GATTGTAGCCATGGATTCTGT |
Primers used for real-time RT-PCR of sigma 70 and rteB
|
|
Sigma 70 FP |
ACGCTGTATGGTGGATTCGTCAGT |
Sigma 70 RP |
ACCTGATTCAACGGGAGACGAACA |
F-rteB-RT |
TGGTAGCCGCCACCGACATGAG |
R-rteB-RT |
CCGCAGTCCACCGCCACAAATG |
Primers used to amplify rteB for pRteB |
|
F-RteB-D55E-NdeI |
ATTGCTTAATCCATATGGATAAGACA |
R-D55E-HindIII-RteB |
CAAATTCAGTGAAGCTTGGGCTATGATTGC |
Primers used for site-directed mutagenesis of rteB
|
|
RteB-D55E-F |
ATCGTGGTTGCCGAACTGCGTCTGCCTGAC |
GC-RteB-D55E-F |
GTCAGGCAGACGCAGTTCGGCAACCACGAT |